ULTRASTRUCTURE OF CARDIOMYOCYTES AND THEIR HISTOPHYSIOLOGICAL CHANGES UNDER HYPOXIC CONDITIONS
Keywords:
cardiomyocyte, ultrastructure, sarcomere, mitochondria, T-tubuleAbstract
This article provides a systematic overview of the ultrastructural organization
of cardiomyocytes (heart muscle cells), their functional characteristics related to
energy and ion-calcium homeostasis, and the histological, ultrastructural, and
physiological changes that occur under conditions of hypoxia and ischemia. The
ultrastructure of the cardiomyocyte’s contractile apparatus (sarcomere), mitochondria,
sarcoplasmic reticulum, transverse tubules (T-tubules), intercalated discs, and nuclear
apparatus is analyzed. The molecular mechanisms of hypoxia (the HIF signaling
pathway, the shift to anaerobic glycolysis, the disruption of Na⁺/Ca²⁺ homeostasis,
and the mitochondrial permeability transition pore) are examined, along with the
ultrastructural markers of reversible and irreversible injury, necrosis and apoptosis,
reperfusion injury, the states of myocardial "hibernation" and "stunning," and
adaptive responses to chronic hypoxia. This article is intended to serve as a
theoretical foundation for advanced-level research (doctoral and post-doctoral
studies)
References
1. Bergmann O., Bhardwaj R.D., Bernard S., et al. Evidence for cardiomyocyte
renewal in humans. Science. 2009;324(5923):98–102. 2. Eisner D.A., Caldwell J.L., Kistamás K., Trafford A.W. Calcium and
excitation-contraction coupling in the heart. Circulation Research. 2017;121(2):181–195.
3. Hausenloy D.J., Yellon D.M. Myocardial ischemia-reperfusion injury: a
neglected therapeutic target. Journal of Clinical Investigation. 2013;123(1):92–
100. 4. Hong T., Shaw R.M. Cardiac T-tubule microanatomy and function. Physiological Reviews. 2017;97(1):227–252. 5. Jennings R.B., Reimer K.A. The cell biology of acute myocardial ischemia. Annual Review of Medicine. 1991;42:225–246. 6. Kalogeris T., Baines C.P., Krenz M., Korthuis R.J. Cell biology of
ischemia/reperfusion injury. International Review of Cell and Molecular
Biology. 2012;298:229–317. 7. Kloner R.A., Jennings R.B. Consequences of brief ischemia: stunning, preconditioning, and their clinical implications. Circulation. 2001;104(24):2981–2989. 8. Murry C.E., Jennings R.B., Reimer K.A. Preconditioning with ischemia: a
delay of lethal cell injury in ischemic myocardium. Circulation. 1986;74(5):1124–1136. 9. Semenza G.L. Hypoxia-inducible factors in physiology and medicine. Cell. 2012;148(3):399–408. 10.Thygesen K., Alpert J.S., Jaffe A.S., et al. Fourth universal definition of
myocardial infarction (2018). Circulation. 2018;138(20):e618–e651.
